Soil biodiversity DNA archaea

The dataset includes archaeal 16S raw DNA sequences for 885 samples collected as part of LUCAS 2018 Soil survey (Biodiversity module).
Registration is requested: 
Yes
Year: 
2026
Keywords: 

Title: Soil biodiversity - DNA archaea.
Description: The dataset includes archaeal 16S raw DNA sequences1 for 885 samples collected as part of LUCAS 2018 Soil survey2,3 for the biodiversity module. Raw DNA sequences are deposited on NCBI Sequence Read Archive (SRA) database under BioProject ID PRJNA1118194. Samples from four locations were re-sampled, and thus sequenced twice, due to sampling issues (i.e., 885 total samples from 881 sampling sites): samples n° 59, 102, 113, 116 were re-sequenced as samples n° 138, 601, 717, 258 respectively.

Sampling site metadata can be found at the Soil Biodiversity - DNA Bacteria and Fungi data and the Soil Biodiversity - DNA Eukaryotes with further details on data acquisition in Labouyrie et al. 20234 and Köninger et al. 20235. To combine single file of DNA sequences with data in the metadata tables, one needs to take the number (from 1 to 885) in the file name and match it to the corresponding “BARCODE_ID” (numbers from 1 to 885) in the table.

Additional details on the DNA extraction, amplification and sequencing can be found in Bahram et al. 2026 “Intensive land use enhances soil ammonia-oxidising archaea at a continental scale1.

Spatial coverage: EU27+ UK
Type:  DNA sequences
Temporal coverage:  2018
 

References: 

  1. Bahram, M., Lehtovirta-Morley, L., Mikryukov, V., Sveen, T.R., Grant, A., Pent, M., Hildebrand, F., Labouyrie, M., Köninger, J., Tedersoo, L., Jones, A., Panagos, P., Orgiazzi, A. 2026. Intensive land use enhances soil ammonia-oxidising archaea at a continental scale. Soil Biology and Biochemistry, 213: 110024.
  2. Orgiazzi, A. et al. LUCAS Soil, the largest expandable soil dataset for Europe: a review. Eur J Soil Sci 69, 140–153 (2018).
  3. Orgiazzi, A. et al. LUCAS Soil Biodiversity and LUCAS Soil Pesticides, new tools for research and policy development. European J Soil Science 73 (2022).
  4. Labouyrie, M., Ballabio, C., Romero, F., Panagos, P., Jones, A.,  Schmid, M., Mikryukov, V., Dulya, O., Tedersoo, L., Bahram, M., Lugato, E., van der Heijden, M.G.A., Orgiazzi, A. 2023. Patterns in soil microbial diversity across EuropeNature Communications14, Article number: 3311, doi: 10.1038/s41467-023-37937-4.
  5. Köninger, J. Ballabio, C., Panagos, P., Jones, A., Schmid, M.W., Orgiazzi, A, Briones, M.J.I. 2023. Ecosystem type drives soil eukaryotic diversity and composition in Europe . Global Change Biology29(19): 5706-5719. DOI: 10.1111/gcb.16871

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